Process of making preventive and curative lymph.



tinrrnn saunas s enna onnicn SIMON KRAFFT, F MUNICH, GERMANY.

PROCESS 0F MAKING PREVENTIVE AND CURATIVE LYMPH.

ital 2 A23.

No Drawing.

To all whom it may concern:

Be it known that L SIMON KRAFFT, doctor of philosophy, a subject of the Emperor of; Germany.residing at Schuherstrasse, Munich, Kingdom of Bavaria, German Empire, have invented certain new and useful Improvements in Processesv for the Mann: t'actnre oi liyinphs for Preventive and Curt tivc llmposes, of which the follow-"lug is a.

specification. I

the present. invention consists-of a new process for the manufacture of lymphs, and

tieularly apt. for obtaining curative and preventive means against hemorrhagic septicemia, swine. plague, and hog cholera.

Hitherto, in attempting to cure animals afilicted with swine plague and hog-cholera and to prevent the spread of such contagious diseases, cultures from diseased animals have repeatedly been cn'mloyed. but with the attainment of but little or no success. According to the present process for obtaining lymphs for curative and preventive purposes, tirstly, a culture of bacteria is ob taincd by allowing a species of bacteria to develop in a suitable nutritive medium, then a heavy metal, or a mixture of several metals, is added, and the metal or metals allowed toact; a certain time, depending on the disease. to be treated and its correspondll'lfj bacteria. The temperature most suitable for the action corresponds with the temperature which is the most favorable. ij'or'thc dovi-Iopinent and growing of the bacteria. The inventor has made the discoverythatcertain metals have on living bacteria. a certain inthicnce, with thepractical result. of import in; to the bacteria product. a curative 0r immunizing power, whereas without such treatment. the bacteria could act; only in juriously. He has found that the beneficial result lies in the fact that by the treatment with metals a peculiar change has 'bee rnct Specification of Letters Patent.

Patented Nov. 30, 1999.

Application filed JuneZZ, 1908. Serial No. 439,841.

fectcd in thcbacteriacells. The length of time necessary in the'practice of the process for the metals to actv on any bacteria: group may be fixed by meansof experiments on animals which would be injured by the untreated or unchanged bacteria. The heavy metals are. in general, suitable for-the prescut: process, but preferably, metals are er'n-" ployed it he soluble compounds-of which have 'no inplrious action on the sub ect which it.

is desired to treat with the lymph. in accordance with the process ftllllllllg' the present. invention, a lymph is nninufactured by the action of a heavy metal. such as zinc, on cultures othighly virulent swine-plague and hog-cholera bacteriiuwhieh'lymplnwhen subcutaneously injected in quantities varying from 5 1025 cubic centimeters. accordingjo the size of the animal. cures feverinfected swine. The product is equally ellicacious as a preventive lymph.

The following is a speciticcxample of the manner in which this lymph may be ob-.

taincd. A culture of virulent swine plague or hog cholera bacteria is obtained by introducing the bacteria of these groups into n00 cubic centimeters of culture bouillon, which is preferably the so-ealled nutritive gelatin, and allowing the same to act for two days in a suitable incubating vessel at a tour perature of 25 C. The swine plague or hog choleraculture is then mixed with 7.5 grams of coarsely cut, chemically pure zinc, which has been previouslv subjected to dry sterilization for about fifteen minutes, and the mixture is placed in the incubating vessel. Here it is maintained at. a-telnperaturc of 22 C. for a period of 15 hours while using the agitatingmechanism. Then 25 grams o't glycerin as pure as possible, and 2- grams of chemically pure carbolic acid are added, a'iittir'whichthe liquid is allowed to settle somewhat and. is filtered. It is to be understood that the carbolic acid has no curative power whatever in the present case, but is added only for the purpose of preventing decomposition of the lymph during a long storage. If it is desired to. use the lymph soon, or immediately after its preparation, the addition of carholic acid may be omitted, if desired.

The quantitative proportions vary according to the conditions. Generally speaking 7.5 gramsofzinc or copper are used, 10 grains of tin'and 5.5 grams of silver, in 500 cubic centimeters of bacteria culture. liquid,

but' larger and smaller quantities can be used, however. The metals are preferably used in the form of powder, or small pieces.

Having thus described my invention, what I claim is 1. The herein described process for the manufacture of lymphs for'preventive and curative purposes, which consists in preparing a culture of a bacteria group, adding thereto a metal and after the reaction is complete, separating the metal from the resulting product, thereby obtaining the curative lymph.

2. The herein described process for the manufacture of lymphs for preventive and curative purposes, which consists in preparing a culture of bacteria of the swine-plague and hog-cholera group, adding thereto a heavy metal the soluble compounds of which do not injure the animal organism, and after the reaction is complete, separating the metal'from the resulting product, thereby obtaining the curative lymph.

3. The herein described process for the manufacture of lymphs for preventive and curative urposes, which consists in preparing a cu ture in nutrltlve gelatin of bacteria of the hog-cholera or swine-plague group, adding thereto a heavy metal, allowing the mixture to act for about fifteen hours in an incubating vessel, allowing to settle and separating the metal from the resulting product, thereby obtaining the curative lymph, substantially as described.

4. The herein described process for the production of lymphs for preventive and curative purposes, which consists in adding bacteria of the swine plague or hog cholera group, to nutritive gelatin, allowing the mixture to act for two days at a temperature of about 25 (3., adding thereto pure zinc in a suitable state of division, allowing the mixture to act for 15 hours at a temperature of about 22 (1., meanwhile agitating the mixture, then adding glycerin and carbolic acid to the mixture, al-lowin the liquid to settle, and then finally filtering the liquid, the metal being separated .from the culture liquid thereby obtaining the curative lymph, substantially as described.

In witness whereof I have hereunto set my hand in presence of two witnesses.

SIMON KRAFFT. Witnesses:

LOUIS F. MUELLER, MATHILDE K. HELD. 

